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[Evaluation of the micro-histological technique for blood clot paraffin embedding in diagnosis by fine-needle
1Servizio di Anatomia e Istologia Patologica, Ospedale S. Maria delle Croci di Ravenna.
Abstract:
Since a certain amount of blood is nearly always present in the material obtained through needle-aspiration, we have considered it helpful to take advantage of this event, in order to increase the information which is drawn from this type of investigation. We have evaluated the material obtained by means of ultrasound guided fine-needle aspiration of 287 lesions of superficial and deep sites, and we have used 1) a microhistologic method, which employs formalin fixation and paraffin embedding of blood clot (while the blood acts as a support of cells and tissue fragments), and 2) the classic smear cytologic method. The percentage of diagnostic accuracy was similar using both methods, but the microhistologic method presented a few advantages, i.e. greater diagnostic reliability, and long preservation of the residual material, which can subsequently be used for further special stains. These considerations are particularly true for hepatic lesions, which are more than a half of all cases.
Insights
A new microhistologic method using blood clot from fine-needle aspiration (FNA) offers improved diagnostic reliability and material preservation compared to classic smear cytology. This technique is particularly beneficial for analyzing hepatic lesions.
Area of Science:
- Cytopathology
- Histopathology
- Medical Imaging
Background:
- Needle-aspiration biopsy often yields samples with blood contamination.
- Leveraging this blood component can potentially enhance diagnostic yield.
- Ultrasound-guided fine-needle aspiration (UG-FNA) is a common diagnostic procedure.
Purpose of the Study:
- To evaluate the utility of a microhistologic method using blood clot from UG-FNA samples.
- To compare the diagnostic accuracy and advantages of the microhistologic method against the classic smear cytologic method.
- To assess the applicability of the microhistologic method for various lesion types, including hepatic lesions.
Main Methods:
- Ultrasound-guided fine-needle aspiration (UG-FNA) was performed on 287 superficial and deep lesions.
- Samples were analyzed using two methods: 1) a microhistologic technique involving formalin fixation and paraffin embedding of blood clot, and 2) the classic smear cytologic method.
- Diagnostic accuracy percentages were compared between the two methods.
Main Results:
- Both the microhistologic and classic smear cytologic methods demonstrated similar percentages of diagnostic accuracy.
- The microhistologic method offered advantages including greater diagnostic reliability.
- The microhistologic method allows for long-term preservation of residual material for further special stains, especially noted for hepatic lesions.
Conclusions:
- The microhistologic method utilizing blood clot from UG-FNA is a valuable technique.
- This method provides enhanced diagnostic reliability and material preservation compared to conventional smear cytology.
- The microhistologic approach is particularly advantageous for the cytopathological evaluation of hepatic lesions.