Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Three-dimensional reconstruction from random projections: orientational alignment via Radon transforms

M Radermacher1

  • 1Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.

Ultramicroscopy
|February 1, 1994
PubMed
Summary

A novel alignment method enhances 3D reconstruction from electron microscopy. This technique accurately refines projection angles, even with low signal-to-noise ratios, improving structural biology insights.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Retrospective investigation of anti-VEGF treatment reality and effectiveness in patients with neovascular age-related macular degeneration (AMD) in Germany: treatment reality of ranibizumab for neovascular AMD in Germany].

Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·2015
Same author

Comparing simple root phenotyping methods on a core set of rice genotypes.

Plant biology (Stuttgart, Germany)·2013
Same author

3D Reconstruction of a Subcomplex of NADH-ubiquinone-oxidoreductase (Complex I) from Yarrowia lipolytica.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2012
Same author

The structure of eukaryotic and prokaryotic complex I.

Journal of structural biology·2009
Same author

New strategies for determining ribosomal subunit structure from electron micrographs by single particle averaging methods.

Biophysical journal·2009
Same author

DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy.

Journal of structural biology·2009

Area of Science:

  • Structural biology
  • Cryo-electron microscopy

Background:

  • Accurate 3D reconstruction from electron microscopy is crucial for understanding molecular structures.
  • Aligning projection images with unknown orientations presents a significant challenge in this process.

Purpose of the Study:

  • To develop and validate a new method for aligning projection images with unknown directions for 3D reconstruction.
  • To refine existing reconstruction techniques by incorporating unknown projection angles.

Main Methods:

  • Developed a novel alignment method combining the Single Exposure Conical Reconstruction Technique (SECReT) with a 3D orientation search.
  • Applied the technique to 3D reconstruction of 50S ribosomal subunits from Escherichia coli.
  • Tested algorithm reliability with model data, including low signal-to-noise ratios.

Related Experiment Videos

Main Results:

  • Successfully aligned projections with unknown directions towards a 3D reference.
  • Refined reconstructions obtained with SECReT by accurately determining projection angles.
  • Demonstrated reliable performance even with signal-to-noise ratios below 1.

Conclusions:

  • The developed method effectively aligns projections with unknown orientations for improved 3D reconstruction.
  • This technique enhances structural biology studies by enabling reliable reconstructions from challenging datasets.
  • The algorithm shows robustness in low signal-to-noise conditions, expanding its applicability.