Suspension organ culture of the fetal mouse ear

To observe the development of the fetal ear, we grew the explanted ears of mouse fetuses 13 days old in suspension organ culture on a roller device for 48 hours. We used the BGJb-medium (Fitton-Jackson Modification) supplemented by 25% fetal bovine serum, and added a solution containing penicillin-streptomycin at the rate of 1% to the total volume. It was observed histologically that some explants developed ossicles that corresponded with those of mouse fetuses 15 days old in vivo. Sensory structures in the inner ear developed in organ culture as well. This method may help to resolve the question of ontogenesis of the ossicles.