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Alpha-amylase production in lactose medium by Bacillus circulans ACB
A Bandyopadhyay1, S C Pal, S K Sen
1Department of Botany, School of Life Sciences, Visva-Bharati University, Santiniketan, India.
Microbiologia (Madrid, Spain)
|December 1, 1993
Abstract:
Alpha-amylase production by Bacillus circulans ACB was studied in various cultural conditions. During nutrient optimisation, it was found that 2% lactose can be utilized by the strain as source of carbon providing better growth and enzyme yields than starch. Ammonium sulfate of the basal medium can be replaced by ammonium nitrate for better growth and alpha-amylase activity. The strain demonstrated significant enhancement in alpha-amylase production when grown at pH 6.6.