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A 60-kDa thymic epithelial cell surface protein as a potent molecule mediating the cellular interaction with immature
T Takeuchi1, H Kuzuhara, H Tamura
1Division of Immunology, National Institute of Neuroscience, NCNP, Tokyo, Japan.
Abstract:
A unique 60-kDa surface protein expressed on the thymic epithelial cells was characterized as a potent molecule participating in the interaction between thymic stromal cells (TSC) and immature T cells. Previously, we reported an athymic mouse-derived T cell clone, N-9F, which proliferates on TSC. In the present study, we established a TSC clone, SL10.3, from a BALB/c mouse. SL10.3 has an epithelial cell nature and supports N-9F and fetal thymocytes growth in vitro. The two rat monoclonal antibodies, AS19 and AS32, directed to the SL10.3 cell surface inhibited N-9F and fetal thymocytes growth on SL10.3, suggesting that the reactive molecule may mediate the cellular interaction between TSC and immature T cells. Both antibodies are directed to the same 60-kDa protein with a pI point of 5.4, but to different epitopes on the protein. The 60-kDa protein is distributed on thymic epithelial cells, fibroblasts, and vascular endothelial cells, but not on the hematopoietic cells tested.