Related Experiment Videos
Effect of T cells on the complement production by monocytes
H Tsukamoto1, K Nagasawa, Y Ueda
1First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Cellular Immunology
|February 1, 1993
Summary
T cells regulate monocyte complement production, reducing C3 and increasing C4. Activated CD4+ T cells, particularly via IFN-gamma and direct interaction, significantly influence these complement factors.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monocytes are key immune cells involved in complement production.
- T cell regulation of monocyte complement synthesis is not fully understood.
Purpose of the Study:
- To investigate the role of T cells in regulating C3 and C4 production by monocytes.
- To elucidate the mechanisms, including humoral factors and direct cell contact, involved in T cell-mediated complement regulation.
Main Methods:
- Co-culture of T cells and monocytes.
- Use of T-cell-conditioned medium (T-CM) and transwell inserts.
- Employing blocking antibodies against cytokines (IFN-gamma) and cell surface molecules (CD2, LFA-1, HLA-DR).
Main Results:
- T cells reduced C3 and enhanced C4 production by monocytes in a dose-dependent manner.
- Activated T cells, especially CD4+ T cells, showed more pronounced effects.
- Interferon-gamma (IFN-gamma) mediated the suppressive effect on C3 and partially the stimulatory effect on C4.
- Direct cell-to-cell contact, involving CD2, LFA-1, and HLA-DR interactions, also modulated C3 and C4 production.
Conclusions:
- T cells regulate monocyte C3 and C4 production through both soluble factors like IFN-gamma and direct cell interactions.
- CD4+ T cells play a significant role in this regulatory process.