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Synenkephalin processing in embryonic rat brain

M I Rodriguez Vida1, M C Kleid, A Ase

  • 1Instituto de Investigaciones Medicas, Seccion Sustancias Vasoactivas, Buenos Aires, Argentina.

Brain Research. Developmental Brain Research
|February 18, 1994
PubMed
Summary
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Synenkephalin processing differs between embryonic and adult rat brains. Embryonic brains show extensive cleavage to a 1.0 kDa peptide, while adult brains retain more intact synenkephalin and yield met-enkephalin.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • Synenkephalin (proenkephalin 1-70) is processed differently in various tissues.
  • The pre-proenkephalin gene is expressed in embryonic rat brain during proliferation.

Purpose of the Study:

  • To investigate and compare synenkephalin processing in embryonic (E18) versus adult rat brains.
  • To characterize the molecular weight forms and processing intermediates of synenkephalin.

Main Methods:

  • Radioimmunoassay (RIA) for immunoreactive (IR)-synenkephalin.
  • Gel filtration chromatography (Sephadex G-50).
  • Affinity column chromatography and High-Performance Liquid Chromatography (HPLC).

Main Results:

Related Experiment Videos

  • Adult rat brains had higher total IR-synenkephalin concentrations than embryonic brains.
  • Embryonic brains showed predominant processing to a 1.0 kDa peptide, comprising 45% of total immunoreactivity.
  • Adult brains contained more intact 8 kDa synenkephalin and partially processed peptides, with IR-met-enkephalin being largely cleaved.

Conclusions:

  • Synenkephalin processing is developmentally regulated in the rat brain.
  • Embryonic brain exhibits extensive cleavage of synenkephalin, yielding a distinct low molecular weight peptide.
  • Adult brain processing results in more intact synenkephalin and free met-enkephalin.