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Hepatocyte aggregate culture technique for bioreactors in hybrid liver support systems
J C Gerlach1, K Klöppel, C Müller
1Chirurgische Klinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
The International Journal of Artificial Organs
|December 1, 1993
Abstract:
Utilizing a modified culture technique for hepatocytes, a high performance suspension culture is possible in which hepatocytes spontaneously form cell aggregates. The aggregates of 20-100 cells have been histologically confirmed to hold a three-dimensional structure, they show a long-term external metabolism and a survival time comparable with standard adhesion cultures. This technique has several advantages in the construction of large scale bioreactors for hybrid liver support systems.