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Related Experiment Videos

Exercise training does not compensate for age-related decrease in myocardial GLUT-4 content

J L Hall1, R S Mazzeo, D A Podolin

  • 1Institute of Pharmacology, Syntex Discovery Research, Palo Alto, California 94303.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|January 1, 1994
PubMed
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Aging significantly reduces heart glucose transporter (GLUT-4) levels in rats. Endurance exercise training did not alter these age-related decreases in myocardial GLUT-4 protein.

Area of Science:

  • Cardiovascular Physiology
  • Metabolic Regulation
  • Aging Research

Background:

  • Insulin-regulatable glucose transporter 4 (GLUT-4) plays a crucial role in myocardial glucose uptake.
  • Age-related changes in cardiac metabolism may impact glucose transport.
  • The effects of endurance exercise on cardiac GLUT-4 in aging are not well understood.

Purpose of the Study:

  • To investigate the impact of aging and endurance exercise training on myocardial GLUT-4 protein and mRNA levels in Fischer 344 rats.
  • To determine if endurance exercise can mitigate age-associated declines in cardiac GLUT-4.

Main Methods:

  • Male Fischer 344 rats of 7, 15, and 25 months of age were subjected to 10 weeks of treadmill running (75% maximal capacity, 1 hr/day, 5 days/wk).
  • Myocardial GLUT-4 protein concentration was measured using SDS-PAGE and Western blotting.

Related Experiment Videos

  • Myocardial GLUT-4 mRNA levels were quantified via slot-blot analysis.

    • Main Results:

    • Myocardial GLUT-4 protein concentration significantly decreased with age, showing a 27% reduction in 25-month-old rats compared to 7-month-old rats.
    • GLUT-4 mRNA levels also decreased in older rats (25-month-old) compared to younger rats (7-month-old).
    • Endurance training did not significantly alter myocardial GLUT-4 protein levels across any age group, despite increasing GLUT-4 mRNA in trained 7- and 25-month-old rats.

    Conclusions:

    • Myocardial GLUT-4 protein levels decline significantly with advancing age in male Fischer 344 rats.
    • Ten weeks of endurance exercise training did not affect myocardial GLUT-4 protein levels in aging rats.
    • Age-related decreases in cardiac GLUT-4 protein appear resistant to short-term endurance training.