Related Experiment Videos
Difference in neutralization between lactate dehydrogenase-elevating virus isolated from acutely and chronically
1Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
The Journal of General Virology
|May 1, 1994
Summary
Lactate dehydrogenase-elevating virus (LDV) causes lifelong viremia in mice, even with antiviral antibodies. Chronic infection allows LDV variants to emerge that resist antibody neutralization, explaining viral persistence.
Area of Science:
- Virology
- Immunology
- Infectious Diseases
Background:
- Lactate dehydrogenase-elevating virus (LDV) establishes lifelong viremia in mice.
- Neutralizing antiviral antibodies are produced but fail to clear the infection.
Purpose of the Study:
- To investigate if viral persistence correlates with the emergence of antibody-resistant LDV variants.
- To compare the neutralization sensitivity of LDV from acute versus chronic infections.
Main Methods:
- Comparison of viral particle neutralization using polyclonal and monoclonal anti-LDV antibodies.
- Testing LDV derived from acutely and chronically infected mice.
Main Results:
- LDV from acute infections was efficiently neutralized by antibodies.
- LDV from chronic infections showed reduced susceptibility to neutralization.
- Acquired resistance to neutralization was observed in LDV passaged through chronically infected animals.
Conclusions:
- LDV variants capable of escaping antibody neutralization emerge during chronic infection.
- This immune escape mechanism likely contributes to lifelong viremia in LDV-infected mice.