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Related Experiment Videos

Comparison and evaluation of ticarcillin and carbenicillin using disk diffusion methods

M F Parry, H C Neu

    Antimicrobial Agents and Chemotherapy
    |April 1, 1976
    PubMed
    Summary

    A new 75-mug ticarcillin disk effectively tests susceptibility in Pseudomonas aeruginosa and Enterobacteriaceae. This method improves upon carbenicillin disk testing, accurately identifying strains sensitive to ticarcillin.

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    Area of Science:

    • Microbiology
    • Clinical Pharmacology

    Background:

    • Carbenicillin disk susceptibility testing is unreliable for predicting ticarcillin activity against Pseudomonas aeruginosa.
    • Ticarcillin demonstrates significantly higher activity against Pseudomonas aeruginosa compared to carbenicillin.

    Purpose of the Study:

    • To evaluate the efficacy of a 75-mug ticarcillin disk for susceptibility testing of P. aeruginosa and Enterobacteriaceae.
    • To establish reliable zone size breakpoints for ticarcillin susceptibility testing.

    Main Methods:

    • Agar dilution studies were performed to determine minimal inhibitory concentrations (MICs).
    • Susceptibility testing was conducted using 50-mug and 100-mug carbenicillin disks, and a 75-mug ticarcillin disk.
    • Correlation between disk diffusion zone sizes and agar dilution MICs was analyzed.

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    Main Results:

    • A 100-mug carbenicillin disk inaccurately classified 43% of P. aeruginosa strains as resistant to ticarcillin.
    • The 75-mug ticarcillin disk showed excellent correlation between zone size and MIC values.
    • A zone size of >= 15 mm indicated susceptibility, while 12-14 mm indicated intermediate susceptibility for both P. aeruginosa and Enterobacteriaceae.

    Conclusions:

    • The 75-mug ticarcillin disk is a reliable and discriminatory method for testing ticarcillin susceptibility in P. aeruginosa and Enterobacteriaceae.
    • This disk diffusion method provides accurate identification of susceptible and resistant strains, improving clinical treatment decisions.