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Detection of carboxyhemoglobin using Fourier transform infrared microspectroscopy
Abstract:
We investigated the absorption of carboxyhemoglobin (COHb) using a Fourier transform infrared (FTIR) microscopy system. Weak absorption at 1969 cm-1 due to Fe<--CO and strong absorption due to N-H stretching vibration, > C = O stretching vibration and -NH bending vibration (1544 cm-1) were observed. The ratio of absorbance at 1969 cm-1/1544 cm-1 can be used as an index of CO saturation. Therefore, when formalin-fixed blood remains in blood vessels in formalin-fixed tissues, the amount of COHb can be calculated using this FTIR microscopy system.