Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methionine transamination--metabolic function and subcellular compartmentation

P W Scislowski1, K Pickard

  • 1Rowett Research Institute, Bucksburn, Aberdeen, Scotland.

Molecular and Cellular Biochemistry
|December 8, 1993
PubMed
Summary

Enzymatic activities for converting L-methionine and 4-methylthio-2-oxobutyric acid (2,4-KMB) were found in rat and sheep tissues. Liver cytoplasm and mitochondria showed the highest activity, suggesting roles in methionine synthesis and disposal.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blood, no sweat and no tears: a novel approach to teaching transfusion-associated circulatory overload.

Transfusion medicine (Oxford, England)·2019
Same author

miR-19-Mediated Inhibition of Transglutaminase-2 Leads to Enhanced Invasion and Metastasis in Colorectal Cancer.

Molecular cancer research : MCR·2015
Same author

FPs and Hospitals.

Canadian family physician Medecin de famille canadien·2011
Same author

MicroRNAs: key players in carcinogenesis and novel therapeutic targets.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2008
Same author

Selenoprotein expression in endothelial cells from different human vasculature and species.

Biochimica et biophysica acta·2002
Same author

Selenite protects human endothelial cells from oxidative damage and induces thioredoxin reductase.

Clinical science (London, England : 1979)·2001

Area of Science:

  • Biochemistry
  • Animal Physiology
  • Metabolic Pathways

Background:

  • L-methionine is an essential amino acid crucial for protein synthesis and methylation.
  • 4-methylthio-2-oxobutyric acid (2,4-KMB) is an analogue of methionine involved in its metabolism.
  • Understanding the enzymes that inter-convert these compounds is vital for metabolic research.

Purpose of the Study:

  • To investigate the enzymatic activities responsible for the inter-conversion of L-methionine and 2,4-KMB in mammalian tissues.
  • To determine the tissue distribution and subcellular localization of these enzymatic activities in rats and sheep.
  • To elucidate the physiological and nutritional roles of these transamination pathways.

Main Methods:

  • Enzyme assays were performed on tissue homogenates from laboratory rat and sheep liver, skeletal muscle, and heart.

Related Experiment Videos

  • Subcellular fractionation was used to localize enzymatic activities within cytoplasmic and mitochondrial compartments.
  • Kinetic parameters were analyzed to understand the catalytic properties of the enzymes involved.

    • Main Results:

    • Enzymatic activities for L-methionine and 2,4-KMB inter-conversion were detected in liver, skeletal muscle, and heart of both species.
    • The highest methionine transamination activity was observed in the liver.
    • These activities were localized to both the cytoplasm and mitochondria in liver tissue.

    Conclusions:

    • Cytoplasmic methionine transamination likely plays a role in the synthesis of L-methionine from 2,4-KMB, supporting nutritional requirements.
    • Mitochondrial activity is proposed to be involved in the disposal of excess methionine via oxidative decarboxylation of 2,4-KMB.
    • These findings highlight distinct roles for methionine metabolism in different subcellular compartments.