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Published on: May 21, 2014
NGF-induced stabilization of GAP-43 mRNA is mediated by both 3' untranslated region and a segment encoding the
1Department of Neurobiology, Faculty of Medicine, University of Tokyo, Japan.
Abstract:
NGF stabilizes cytoplasmic mRNA for GAP-43, a neuronal growth cone protein. A 292 b segment of rat GAP-43 mRNA was determined to mediate this stabilizing effect. Insertion of the 292 b segment into heterologous mRNA was also shown to constitutively destabilize the mRNA in the absence of NGF. This 292 b segment contains the sequences encoding the carboxy-terminus peptide and part of the 3' untranslated region. Neither one of these sequences can fully mediate the NGF-dependent stability control independently from each other. Premature termination of translation before the 292 b segment reduces both the constitutive destabilization by the 292 b segment and the NGF-induced stabilization. These findings indicate that the constitutive destabilizing function of the 292 b segment is dependent on the direct translation of this segment and that NGF suppresses the destabilizing function of this segment.
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