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Updated: Jul 29, 2026

Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
Published on: November 22, 2017
Relationship between sperm nucleus remodelling and cell cycle progression of fragments of mouse parthenogenotes
M S Szöllösi1, E Borsuk, D Szöllösi
1I.N.R.A., Unité de Biologie de la Fécondation, Station de Physiologie Animale, Jouy-en-Josas, France.
Abstract:
Nucleate and anucleate fragments of parthenogenetically activated mouse oocytes, as well as cybrids obtained by fusion of anucleate fragments (cytoplasts) of maturing and activated matured oocytes were fertilized at different time after activation. Remodelling of the sperm nucleus was studied by electron microscopy at 1.5 and 3 h after fertilization and, in addition, at 14 h in cybrids. Results show that 1) the nuclear envelope of the sperm nucleus can break down when the insemination takes place after the end of M-phase, but the capacity of the parthenote cytoplasm to remodel the sperm nucleus is restricted in time. 2) Male chromatin can decondense within the old, unbroken nuclear envelope, but in such cases formation of a male pronucleus, one of the two nuclei of zygote possessing inactive nucleoli, is never observed.
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