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UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus
1Institut für Mikrobiologie und Biochemie, Friedrich-Alexander Universität Erlangen-Nürnberg, FRG.
FEMS Microbiology Letters
|April 1, 1994
Abstract:
We have analyzed the sequence downstream of rpoN from Acinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP-N-acetylglucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus. The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN. These observations confirm that A. calcoaceticus represents the first exception from a conserved genetic context of rpoN observed in several other Gram-negative bacteria.