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Isolation and sequence of the t-RNA ligase-encoding gene of Candida albicans
J Baymiller1, S Jennings, B Kienzle
1Department of Microbial Molecular Biology, Bristol Myers Squibb Pharmaceutical Research Institute, Princeton, NJ 08543.
Abstract:
The gene encoding tRNA ligase from Candida albicans was isolated from a genomic library by complementation of a Saccharomyces cerevisiae strain containing a disrupted structural gene, RLG1, encoding tRNA ligase. The cloned gene also complements a temperature-sensitive allele of RLG1. Sequence analysis revealed a single 2499-nt coding region. The gene encodes a protein of 833 amino acids that is 42% identical to S. cerevisiae tRNA ligase. Hybridization to chromosomes of C. albicans separated by pulsed-field gel electrophoresis located the gene to chromosome 1, the smallest C. albicans chromosome.