Immunofluorescence and immunoperoxidase observations of anti-lactic dehydrogenase-1 antibody

For immunoelectron-microscopic demonstration of lactic dehydrogenase (LDH), anti-subunit-B antibody was produced in rabbits by immunization with purified pig LDH-1 (B4) isoenzyme. Immunoglobin fragment Fab' was obtained from anti-LDH serum immunoglobulin G and conjugated with horseradish peroxidase. Subunit-B-containing LDH was present diffusely in the sarcoplasm of the pig heart muscle. There was concentration of LDH in the 1-band. LDH reaction was also present in the mitochondrial intermembranous space. The present immunoelectron-microscopic examination is in general agreement with the previous cytochemical demonstration of LDH using thiocarbamylnitro blue tetrazolium.