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The Unp proto-oncogene encodes a nuclear protein
K Gupta1, M Chevrette, D A Gray
1Department of Biochemistry, University of Ottawa, Canada.
Oncogene
|June 1, 1994
Summary
The mouse Unp gene, related to the human oncogene TRE17, encodes a novel nuclear oncoprotein. Its expression transforms cells, indicating a role in tumorigenesis.
Area of Science:
- Molecular biology
- Oncology
- Gene expression analysis
Background:
- The mouse Unp gene shares similarities with the human oncogene TRE17 but is not its direct homolog.
- Unp is a ubiquitously expressed gene that generates two distinct messenger RNA (mRNA) transcripts.
Purpose of the Study:
- To investigate the function and oncogenic potential of the mouse Unp gene.
- To determine the cellular localization and transforming activity of the Unp protein.
Main Methods:
- Analysis of Unp gene expression and mRNA variants.
- Determination of Unp protein localization within the cell nucleus.
- Assessment of tumorigenic transformation induced by Unp expression in NIH3T3 cells.
Main Results:
- The Unp protein is confirmed to be localized in the cell nucleus.
- Expression of Unp from a potent promoter induced tumorigenic transformation in NIH3T3 cells implanted in athymic mice.
Conclusions:
- The mouse Unp gene encodes a novel nuclear oncoprotein.
- Unp plays a significant role in cellular transformation and tumorigenesis.