Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Localization and functional role of the pseudomonas bacteriophage 2 depolymerase

F J Castillo, P F Bartell

    Journal of Virology
    |May 1, 1976
    PubMed
    Summary

    Phage 2 depolymerase, an enzyme associated with the phage base plate, plays a key role in phage adsorption and bacterial cell lysis. This enzyme degrades bacterial receptors, facilitating phage entry and release.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Activity of imipenem/relebactam against Enterobacterales and Pseudomonas aeruginosa in Spain. SMART 2016-2020.

    Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2023
    Same author

    Antimicrobial activity of ceftolozane-tazobactam against Enterobacterales and Pseudomonas aeruginosa recovered during the Study for Monitoring Antimicrobial Resistance Trends (SMART) program in Spain (2016-2018).

    Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2021
    Same author

    Monitoring the antimicrobial susceptibility of Gram-negative organisms involved in intraabdominal and urinary tract infections recovered during the SMART study (Spain, 2016 and 2017).

    Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2019
    Same author

    Antimicrobial susceptibility trends and evolution of isolates with extended spectrum β-lactamases among Gram-negative organisms recovered during the SMART study in Spain (2011-2015).

    Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2018
    Same author

    Antioxidative protection in the inducible CAM plant Sedum album L. following the imposition of severe water stress and recovery.

    Oecologia·2017
    Same author

    Evaluation of four phenotypic methods to detect plasmid-mediated AmpC β-lactamases in clinical isolates.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2012

    Area of Science:

    • Bacteriology
    • Molecular Biology
    • Virology

    Background:

    • Phage 2 adsorption involves a complex base plate structure.
    • Phage 2 depolymerase is an induced hydrolytic enzyme linked to the base plate.

    Purpose of the Study:

    • To investigate the role of phage 2 depolymerase in phage-host interactions.
    • To elucidate the function of depolymerase in phage adsorption and bacterial cell lysis.

    Main Methods:

    • Purification and characterization of phage 2 depolymerase.
    • Treatment of bacterial cells and receptor fractions with depolymerase.
    • Analysis of phage adsorption inhibition and bacterial lysis.

    Main Results:

    • Phage 2 depolymerase is a structural protein or associated with the base plate.
    • Depolymerase treatment inhibited phage 2 adsorption to bacterial cells.
    • Hydrolysis of bacterial receptors by depolymerase released amino sugars and abolished phage inactivation.
    • Depolymerase induced lysis of bacterial cells in hypotonic medium.

    Conclusions:

    • Phage 2 depolymerase is crucial for phage adsorption by degrading bacterial receptors.
    • The enzyme facilitates phage release through bacterial cell lysis.
    • A mutant polypeptide (Pdp1) is antigenically similar to depolymerase but lacks hydrolytic activity, localizing to the base plate.

    Related Experiment Videos