Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

DNA fingerprinting in Falconidae

I Rychlík¹, O Kubícek, V Holcák

¹Veterinary Research Institute, Brno.

Veterinarni Medicina

|January 1, 1994

Summary

DNA fingerprinting using the (GTG)5 oligonucleotide probe reveals high polymorphism in falcons. This method effectively differentiates individual birds and confirms relationships within closely related falcon species.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mycobacteriosis: the most common causative agents.

Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne·2023

Same author

Carnosinase Gene-Is It Responsible for Diabetic Nephropathy?: Carnosine as a Protective Factor in Diabetic Nephropathy: Association with a Leucine Repeat of the Carnosinase Gene CNDP1. Diabetes 54: 2320-2327, 2005.

Journal of the American Society of Nephrology : JASN·2023

Same author

Bilateral simultaneous tubal pregnancy.

Ceska gynekologie·2020

Same author

Bladder rupture after a fall on the ground in a patient with total uterine prolapse.

Ceska gynekologie·2019

Same author

Epidemiology of selected Mycobacterium tuberculosis complex members in the Czech Republic in 2000-2016.

Epidemiologie, mikrobiologie, imunologie : casopis Spolecnosti pro epidemiologii a mikrobiologii Ceske lekarske spolecnosti J.E. Purkyne·2019

Same author

Characteristics of high- and low-risk individuals in the PRIORITY study: urinary proteomics and mineralocorticoid receptor antagonism for prevention of diabetic nephropathy in Type 2 diabetes.

Diabetic medicine : a journal of the British Diabetic Association·2018

Area of Science:

Molecular Biology
Genetics
Ornithology

Background:

Accurate identification and differentiation of closely related avian species are crucial for conservation and research.
Traditional methods may lack the resolution to distinguish between individuals or hybrids within species like falcons.

Purpose of the Study:

To evaluate the effectiveness of the oligonucleotide probe (GTG)5 for DNA fingerprinting in falcons.
To assess the potential of this method for differentiating individual falcons, including interspecies hybrids.

Main Methods:

Blood samples from falcons (Falco peregrinus, F. rusticolus, F. cherrug, and hybrids) were processed.
DNA was immobilized, lysed, and digested in low-melting point agarose.
The oligonucleotide probe (GTG)5 was used in conjunction with restriction endonucleases (Hinf I, Hae III, Msp I).

Main Results:

The (GTG)5 probe generated numerous DNA fragments, some genus-specific and others female-specific.
Approximately 5-10% of the fragments were individual-specific, enabling unique identification.
DNA fingerprints confirmed known relationships among individual birds.

Conclusions:

DNA fingerprinting with the (GTG)5 oligonucleotide probe is a powerful tool for avian genetics.
This technique allows for precise differentiation among closely related falcon species and individuals.
The method supports conservation efforts by enabling accurate identification and relationship confirmation.

Related Experiment Videos