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A simple technique for red blood cell removal in major ABO-incompatible bone marrow transplantation
G Mayer1, D Wernet, H Northoff
1Department of Transfusion Medicine, University of Tübingen, FRG.
Vox Sanguinis
|January 1, 1994
Abstract:
A simple technique for red blood cell (RBC) removal in major ABO-incompatible bone marrow transplantation is reported requiring two centrifugation steps, special blood bags and a mechanical device to separate the buffy coat from RBCs within the bag. In 42 transplantations an average of 84% of nucleated cells was recovered with an average contamination of 7.5 ml packed RBCs. The preparations were well tolerated in all patients whose isoagglutinin titers had not been reduced. Bone marrow engraftment was not significantly different from control groups.