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Related Experiment Videos

Morphological changes in the flexuous potato viruses upon decoration in immunosorbent electron microscopy

I D Garg1, S M Khurana

  • 1Division of Plant Pathology, Central Potato Research Institute, Shimla, India.

Acta Virologica
|October 1, 1993
PubMed
Summary

Potato viruses X and S (PVS) showed good virion morphology during antiserum decoration, unlike potato virus Y (PVY) which experienced disorganization. Preserving PVY virion structure required liquid-phase reactions or glutaraldehyde fixation.

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Area of Science:

  • Plant Virology
  • Immunoelectron Microscopy
  • Molecular Plant Pathology

Background:

  • Antiserum decoration is a technique used to study virus morphology and identification.
  • Potato viruses X (PVX), S (PVS), and Y (PVY) are significant pathogens affecting potato crops worldwide.
  • Understanding virion stability during serological techniques is crucial for accurate diagnosis.

Purpose of the Study:

  • To investigate the impact of antiserum titer, pH, and virus-source host on the virion morphology of PVX, PVS, and PVY during antiserum decoration.
  • To determine optimal conditions for preserving virion integrity during serological analysis of these potato viruses.

Main Methods:

  • Decoration of purified PVX, PVS, and PVY virions with specific antisera.
  • Systematic variation of antiserum titer and pH during the decoration process.

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  • Observation of virion morphology using electron microscopy.
  • Testing the effect of virus-source host species on decoration outcomes.
  • Evaluating the efficacy of liquid-phase reactions and glutaraldehyde fixation for preserving PVY virion structure.

    • Main Results:

    • PVX and PVS generally exhibited good virion decoration with preserved morphology, except under specific conditions (pH 6.0, antiserum titer 1:0.5) where decoration was limited.
    • PVY (o strain) virion morphology was susceptible to disorganization, ranging from slight to extensive, influenced by antiserum pH, titer, and virus-source host.
    • Virion structure of PVY was successfully maintained when reactions occurred in the liquid phase or after fixation with 3% glutaraldehyde prior to decoration.

    Conclusions:

    • The stability of potato virus virions during antiserum decoration is variable and depends on the virus species, antiserum properties (pH, titer), and reaction conditions.
    • PVX and PVS are relatively stable under typical decoration conditions, while PVY virions are more sensitive to disorganization.
    • Specific strategies, such as liquid-phase reactions or glutaraldehyde fixation, are necessary to preserve PVY virion morphology for accurate analysis.