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Canine distemper virus replication in cells on microcarriers
1Institute of Virology, Slovak Academy of Sciences, Bratislava.
Acta Virologica
|October 1, 1993
Summary
Gelatin microcarriers support cell growth for large-scale canine distemper virus production. Suspension cultures using these microcarriers yielded over 10 times more virus than stationary cultures.
Area of Science:
- Cell biology
- Virology
- Biotechnology
Background:
- Suspension cell culture offers scalability for biological production.
- Microcarriers are utilized to enhance cell growth in suspension.
- Efficient virus production is crucial for vaccine development.
Purpose of the Study:
- To evaluate Gelaspher M gelatin microcarriers for cell culture.
- To assess the suitability of microcarrier suspension culture for canine distemper virus production.
- To compare virus yields from microcarrier suspension culture versus stationary culture.
Main Methods:
- Chick embryo and Vero cells were cultured on Gelaspher M gelatin microcarriers in suspension.
- Cell morphology and growth were monitored.
- Canine distemper virus was produced in microcarrier cell cultures.
- Virus yields were quantified using TCID50 per ml and compared to stationary cultures.
Main Results:
- Gelaspher M microcarriers did not adversely affect cell morphology or growth.
- Microcarrier suspension cultures supported large-scale canine distemper virus production.
- Virus yields were over 10-fold higher in microcarrier suspension cultures compared to stationary cultures.
Conclusions:
- Gelatin microcarriers are effective for cell culture in suspension.
- Microcarrier-based suspension culture significantly enhances canine distemper virus production.
- This method presents a scalable and efficient approach for virus yield optimization.