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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein

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Apolipoprotein E in hyperlipidemia

C C Walden¹, R A Hegele

¹St. Michael's Hospital, Toronto, Ontario, Canada.

Annals of Internal Medicine

|June 15, 1994

Summary

DNA analysis of apolipoprotein E (APOE) helps identify individuals predisposed to hyperlipidemia, particularly type III hyperlipoproteinemia. Genetic factors and environmental influences interact to impact plasma lipoproteins.

Area of Science:

Genetics
Molecular Biology
Biochemistry

Background:

Apolipoprotein E (APOE) plays a crucial role in lipoprotein metabolism.
Alterations in APOE are linked to various lipid disorders.
Understanding APOE's genetic variations is key to managing hyperlipidemia.

Purpose of the Study:

To review the application of apolipoprotein E DNA analysis in assessing hyperlipidemia patients.
To explore the role of APOE in normal and disordered lipoprotein metabolism.
To examine genetic analysis of APOE in populations and individuals with type III hyperlipoproteinemia.

Main Methods:

Review of 44 basic science studies on molecular analysis of APOE.
Analysis of 42 studies on APOE's biochemical and molecular biological features.

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Inclusion of 29 clinical studies, meta-analyses, and case series on APOE mutations.

Main Results:

Common APOE DNA variants are associated with plasma lipid differences in the general population.
APOE E2 homozygosity predisposes to type III hyperlipoproteinemia, requiring additional factors for expression.
Rare APOE mutations can also lead to hyperlipidemia.

Conclusions:

APOE DNA variation is a significant factor interacting with environmental influences on plasma lipoproteins.
APOE DNA analysis can identify individuals with type III hyperlipoproteinemia.
Genetic predisposition to hyperlipidemia can be identified in relatives using APOE analysis.