Distribution and regulation of rab3C, a small molecular weight GTP-binding protein

We have investigated the expression and regulation of rab3C in rat tissues and rat pheochromocytoma PC12 cells by using a polyclonal antibody that specifically recognizes this protein. Rab3C was expressed in the neuroendocrine systems, as is its highly homologous family member, rab3A. Strong immunoreactivity of rab3C was also observed in testis, heart and 3 different adipose tissues. In addition, induction of neuronal differentiation in PC12 cells, which causes an increase in catecholamine secretion, also led to an increase in rab3C expression. In summary, our data demonstrate a defined pattern of rab3C expression in the rat; such expression is not limited to the neuroendocrine systems. Regulated secretion of various hormones occurs from all of these rab3C-containing tissues. Our results provide important insight into understanding the potential role of rab3C in regulated exocytosis.