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Sex identification by polymerase chain reaction using a Y-autosome homologous primer set
1Department of Human Genetics, School of International Health, University of Tokyo, Japan.
The Japanese Journal of Human Genetics
|December 1, 1993
Abstract:
We have developed a one-step polymerase chain reaction (PCR) technique which detects a sequence on the human Y chromosome and an autosomal sequence in one reaction. The method is very reliable for the sex determination, as the detection of the autosome-specific signal ensures the presence of DNA in the specimen even in the absence of the Y-specific signal.