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Chloramine mutagenesis in Bacillus subtilis

K L Shih, J Lederberg

    Science (New York, N.Y.)
    |June 11, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Chloramine, a water disinfection by-product, is a weak mutagen. Bacterial DNA repair mechanisms may counteract chloramine

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    Area of Science:

    • Microbiology
    • Genetics
    • Environmental Health

    Background:

    • Chloramine is a common disinfection by-product in public water supplies.
    • Understanding its biological effects is crucial for public health.

    Purpose of the Study:

    • To investigate the mutagenic potential of chloramine.
    • To explore the role of DNA repair in bacterial resistance to chloramine.

    Main Methods:

    • Utilized Bacillus subtilis trpC reversion assay to assess mutagenicity.
    • Examined survival rates of wild-type and DNA-repair mutant strains after chloramine exposure.
    • Investigated the impact of plating media on cellular recovery.

    Main Results:

    • Chloramine demonstrated weak mutagenic activity in the bacterial assay.

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  • Certain DNA-repair mutants exhibited increased sensitivity to chloramine.
  • Plating media influenced the survival of chloramine-treated cells, suggesting repair processes.

    • Conclusions:

    • Chloramine possesses weak mutagenic properties.
    • Bacterial DNA repair systems likely play a role in mitigating chloramine's bactericidal effects.
    • Further research into chloramine's genotoxicity and repair mechanisms is warranted.