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Related Experiment Videos

Sympathetic neuroblasts undergo a developmental switch in trophic dependence

S J Birren1, L Lo, D J Anderson

  • 1Division of Biology 216-76, California Institute of Technology, Pasadena 91125.

Development (Cambridge, England)
|November 1, 1993
PubMed
Summary

Sympathetic neuroblasts initially depend on neurotrophin-3 (NT-3) for survival. Later, they switch to nerve growth factor (NGF) dependence, impacting neuronal development.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Sympathetic neurons require nerve growth factor (NGF) for survival.
  • The timing of neurotrophic factor dependence in developing sympathetic neurons is not well understood.

Purpose of the Study:

  • To investigate the initial neurotrophic factor requirements of sympathetic neuroblasts.
  • To correlate neurotrophin receptor expression with functional dependence during development.

Main Methods:

  • Immuno-isolation of mitotically active sympathetic neuroblasts from different embryonic stages.
  • In vitro survival assays in serum-free medium with different neurotrophins.
  • In vivo analysis of neurotrophin receptor mRNA expression (TrkC and TrkA).

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Main Results:

  • Embryonic day 14.5 (E14.5) sympathetic neuroblasts are supported by neurotrophin-3 (NT-3), not NGF.
  • NT-3 acts as a survival factor for E14.5 neuroblasts.
  • By birth, sympathetic neurons are primarily supported by NGF, with weak NT-3 support.
  • A reciprocal switch in TrkC and TrkA mRNA expression correlates with this change in neurotrophin dependence.

Conclusions:

  • Neurotrophic factors influence sympathetic neuron number earlier in development than previously thought.
  • The acquisition of NGF dependence may involve the downregulation of receptors for earlier survival factors like NT-3.