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Role of replaced v-src and env genes in the duck-adapted variant of Rous sarcoma virus
I Hlozánek1, A V Rynditch, A Mikhailik
1Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Praha.
Abstract:
The env and v-src genes of a duck-adapted variant of Rous sarcoma virus were replaced for corresponding genes from parental chick-derived virus. The generation of viral constructs with replaced genes is described. DNAs of viruses with replaced genes were assayed on chick and duck embryo fibroblasts by transfection assays. Transformation efficiency was measured by the focus assay and multiplication of virus by the reverse transcriptase assay. Duck-adapted virus with replaced env gene lost the higher transformation efficiency for duck cells, whereas replacement of the v-src gene had no effect on its host-specific transformation activity.