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Related Experiment Videos

Quantitation in biological X-ray microanalysis, with particular reference to histochemistry

A T Sumner

    Journal of Microscopy
    |September 1, 1978
    PubMed
    Summary

    X-ray microanalysis reveals a linear relationship between element concentration and X-ray counts in gelatine specimens. Corrections for continuum effects ensure linearity even for heavier elements, aiding accurate elemental analysis.

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    Area of Science:

    • Materials Science
    • Analytical Chemistry
    • Physics

    Background:

    • X-ray microanalysis is a powerful technique for elemental composition determination.
    • Quantifying results can be challenging due to factors like continuum radiation and mass loss.
    • Understanding the relationship between X-ray counts and elemental concentration is crucial for accurate analysis.

    Purpose of the Study:

    • To investigate the relationship between X-ray counts and elemental concentration in gelatine-based specimens.
    • To determine if this relationship is linear for both light and heavy elements.
    • To identify and discuss factors affecting quantitative X-ray microanalysis.

    Main Methods:

    • Subjecting thin specimens of various light and heavy elements in gelatine to X-ray microanalysis.

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  • Measuring X-ray counts for specific elements and expressing them as a percentage of the continuum.
  • Applying corrections for continuum effects to analyze heavy elements.

    • Main Results:

    • A strictly linear relationship was observed between percentage counts and concentration for light elements.
    • Heavier elements showed a non-linear relationship due to continuum count increases.
    • After correction for continuum effects, heavy elements also exhibited a linear relationship.
    • Linearity was confirmed for elements up to atomic number 56 and concentrations around 10%.

    Conclusions:

    • X-ray microanalysis of bulk gelatine specimens demonstrates a linear correlation between X-ray counts and elemental concentration.
    • Corrections for continuum effects are essential for accurate heavy element quantitation.
    • Consideration must be given to specimen preparation, continuum radiation, mass loss, and stain effects in histochemical applications.