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Isolation of thyroid peroxidase from patients with Graves' disease and comparison with animal peroxidases
T Hosoya1, H Yoshimura, N Naito
1Faculty of Pharmaceutical Sciences, Chiba University, Japan.
The International Journal of Biochemistry
|March 1, 1994
Abstract:
1. Human thyroid peroxidase (TPO) was isolated from 280-640 g of pooled thyroid tissue resected from patients with Graves' disease. 2. Isolation was performed by an improved and simplified method. 3. The Reinheit Zahl (A412/A280) of the final preparations was in the range of 0.16-0.32. 4. The spectroscopic and enzymatic properties of Graves' TPO were compared with those of porcine TPO and bovine LPO, revealing closer resemblance to the former. 5. Graves' TPO may provide a useful substitute for normal TPO, which is very difficult to isolate.