[Use of Mancini's method of simple radial immunodiffusion]

The sensitivity of Mancini's test of simple radial immunodiffusion depends upon a number of conditions which in certain combinations may increase the sensitivity of the test 4-5-fold. Using empirical formulas a table has been composed stating the sizes of precipitation ring diameters at different concentrations of antiglobulin sera and dilutions of standard sera. Using this table, the optimal concentration of antiglobulin sera in agar may be calculated with minimal number of preliminary tests.