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Related Experiment Videos

Polymorphism at the mouse Thb locus

T P Gumley1, I F McKenzie, M S Sandrin

  • 1Molecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia.

Immunogenetics
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

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The ThB glycoprotein

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Mouse ThB is a cell surface glycoprotein homologous to Ly-6 molecules.
  • The Thb locus has two alleles (Thbh and Thbl) controlling ThB expression levels on thymocytes and B cells.
  • This expression polymorphism differs from typical Ly loci polymorphisms.

Purpose of the Study:

  • To investigate the molecular basis for differential ThB expression between high-expressing (C57BL/6) and low-expressing (BALB/c) mouse strains.
  • To determine if variations in protein structure, glycosylation, or transcriptional control underlie the observed expression differences.

Main Methods:

  • Analysis of the RNA (cDNA) sequence of Thbh and Thbl alleles.
  • Comparison of nucleotide sequences between ThB(high) and ThB(low) expressing strains.

Related Experiment Videos

  • Quantification of ThB mRNA levels in different expressing strains.

    • Main Results:

    • Complete nucleotide identity was found in the cDNA sequences of Thbh and Thbl alleles.
    • RNA and protein sequences of ThB molecules are identical between high and low expressing strains.
    • Significant differences in ThB mRNA levels were observed between ThB(high) and ThB(low) strains.

    Conclusions:

    • The differential expression of ThB glycoprotein is not due to variations in protein or RNA sequence.
    • Differences in ThB expression levels are likely regulated at the transcriptional level.
    • The Thb alleles exhibit distinct transcriptional rates, leading to varied ThB glycoprotein expression.