Related Experiment Videos
Artificial conditioner for stored organs
A Kawamura1, J Meguro, M Takahashi
1Department of Surgery, Sapporo Hokuyu Hospital Artificial Organ & Transplantation Hospital, Japan.
The International Journal of Artificial Organs
|January 1, 1994
Summary
This study introduces an artificial organ conditioning system to assess and improve long-term stored and warm ischemia-damaged kidneys. The system shows promise for evaluating kidney viability after cardiac arrest, potentially enabling transplantation.
Area of Science:
- Transplantation Science
- Organ Preservation
- Biomedical Engineering
Background:
- Kidney grafts stored long-term or damaged by warm ischemia post-cardiac arrest pose transplantation challenges.
- Assessing graft viability after such insults is critical for successful transplantation outcomes.
Purpose of the Study:
- To develop and evaluate an artificial organ conditioning system for damaged and stored kidney grafts.
- To assess the viability of kidneys subjected to warm ischemia and prolonged storage.
Main Methods:
- A conditioning system comprising an artificial lung, roller pump, organ chamber, and specialized perfusate was utilized.
- Continuous perfusion under mild hypothermia (24°C) was employed for conditioning.
- The system was tested on rabbit kidneys with varying durations of warm ischemia.
Main Results:
- Mildly damaged kidneys (0-30 minutes warm ischemia) were effectively conditioned.
- Severely damaged kidneys did not produce urine, indicating limitations in conditioning severe damage.
- The system demonstrated the ability to assess stored kidney viability and condition damaged kidneys.
Conclusions:
- The developed artificial organ conditioning system can evaluate the viability of stored and warm ischemia-damaged kidneys.
- This method shows potential for enabling kidney harvesting after cardiac arrest, expanding donor organ availability.
- Further refinement may improve conditioning of severely damaged organs.