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Related Experiment Videos

Cholesteryl ester transfer between high density lipoprotein and phospholipid bilayers

J R Morrison1, M J Silvestre, R C Pittman

  • 1Department of Medicine, University of California, San Diego, La Jolla 92093-0682.

The Journal of Biological Chemistry
|May 13, 1994
PubMed
Summary

High density lipoprotein cholesteryl esters (HDL CE) are selectively taken up by cells. This study shows HDL CE transfer to membranes occurs via collision, independent of proteins or apolipoproteins.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Lipid Metabolism

Background:

  • High density lipoprotein-associated cholesteryl esters (HDL CE) are taken up by cells through a process termed selective uptake.
  • This pathway involves HDL CE incorporation into the plasma membrane without parallel uptake of HDL apolipoproteins.

Purpose of the Study:

  • To investigate the initial step of selective uptake: the reversible incorporation of HDL CE into the plasma membrane.
  • To determine the role of membrane proteins and apolipoproteins in HDL CE incorporation.

Main Methods:

  • Compared HDL CE incorporation rates into isolated rat liver plasma membranes versus protein-free synthetic membranes.
  • Utilized radiolabeled tracers to quantify CE pools and transfer kinetics.
  • Investigated potential mechanisms including transient fusion using cardiolipin as a membrane coat marker.

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Main Results:

  • Both biological and synthetic membranes showed saturable HDL CE uptake, with similar rates and capacities (2-4 mol % relative to phospholipids).
  • Two distinct CE pools were identified with different half-lives (approx. 0.5 h and 30 h).
  • CE transfer was second-order, unlike unesterified cholesterol transfer (first-order), and did not involve apolipoproteins or HDL/membrane fusion.

Conclusions:

  • HDL CE transfer to membranes is a collision-mediated process.
  • This transfer is independent of membrane proteins and apolipoproteins.
  • The findings elucidate a novel mechanism for lipid transfer in cellular lipid metabolism.