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Effects of Listeria monocytogenes and its components on adenosine triphosphate concentrations in mice
Abstract:
The effects of Listeria monocytogenes and its components on adenosine triphosphate (ATP) concentrations in mice were examined by in vitro and in vivo techniques. White female mice were intraperitoneally injected with L monocytogenes strain 9-125 and its components, and certain tissues of mice were exposed to the various listerial components in vitro, and the ATP concentrations in these tissues were measured. Results of in vivo experiments indicated that live listerial cells and certain components (hemolysin, cell wall, and cytoplasm) decreased the ATP concentrations in some tissues of the mice, whereas other components (peptidoglycan and endotoxin-like material) did not have any effects on ATP concentrations. Although hemolysin from L monocytogenes decreased the tissue ATP concentrations in vitro, other listerial components did not effect any of the exposed tissues. The data indicated some impairment of host energy metabolism occurred in mice exposed to L monocytogenes or to its components or to both.
Insights
Listeria monocytogenes infection and its components reduce adenosine triphosphate (ATP) levels in mice tissues. This suggests Listeria impairs host energy metabolism.
Area of Science:
- Microbiology
- Immunology
- Biochemistry
Background:
- Listeria monocytogenes is a pathogen that can cause severe illness.
- Understanding its impact on host cellular energy is crucial for developing treatments.
Purpose of the Study:
- To investigate the effects of Listeria monocytogenes and its components on adenosine triphosphate (ATP) concentrations in mice.
- To determine how specific Listeria components influence host energy metabolism.
Main Methods:
- In vivo experiments involving intraperitoneal injection of mice with L. monocytogenes and its components.
- In vitro exposure of mouse tissues to listerial components.
- Measurement of ATP concentrations in various tissues using quantitative techniques.
Main Results:
- Live L. monocytogenes cells, hemolysin, cell wall, and cytoplasm significantly decreased ATP concentrations in certain mouse tissues.
- Peptidoglycan and endotoxin-like material from L. monocytogenes did not affect ATP levels.
- In vitro, only hemolysin reduced tissue ATP concentrations, while other components had no effect.
Conclusions:
- Listeria monocytogenes and specific components like hemolysin impair host energy metabolism by reducing ATP levels in mouse tissues.
- The findings highlight the role of Listeria components in disrupting cellular energy homeostasis.