Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of AP-1 function in cellular transformation pathways

T Suzuki1, M Murakami, N Onai

  • 1Department of Tumor Virus Research, University of Tokyo, Japan.

Journal of Virology
|June 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel approach inducing transplant tolerance by activated invariant natural killer T cells with costimulatory blockade.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2014
Same author

Cowden's disease manifested by esophageal polyposis with characteristic appearance on magnifying endoscopy using narrow band imaging.

Endoscopy·2013
Same author

Education and Imaging. Gastrointestinal: gastric mucosa-associated lymphoid tissue (MALT) lymphoma observed by magnifying endoscopy with narrow band imaging.

Journal of gastroenterology and hepatology·2012
Same author

Construction of a model to estimate the CYP3A inhibitory effect of grapefruit juice.

Die Pharmazie·2011
Same author

Clinicopathological factors associated with clinical outcomes of endoscopic submucosal dissection for colorectal epithelial neoplasms.

Endoscopy·2009
Same author

Endoscopic submucosal dissection for early gastric cancer: a large-scale feasibility study.

Gut·2008
Same journal

Dysregulation of miRNAs has broad impacts on virus infection in <i>Drosophila</i>.

Journal of virology·2026
Same journal

Identification of GRP78 as a novel host factor that facilitates zoonotic porcine deltacoronavirus internalization and replication via clathrin-mediated endocytosis.

Journal of virology·2026
Same journal

MGF110-2L deletion prevents IFN-I and inflammatory response, resulting in partial attenuation and protection against virulent ASFV.

Journal of virology·2026
Same journal

Periodic genome sequences facilitate packaging in a single-stranded DNA virus.

Journal of virology·2026
Same journal

A novel monoclonal antibody targeting the hemagglutinin-neuraminidase of peste des petits ruminants virus maintains neutralizing activity by blocking viral adsorption and receptor interaction.

Journal of virology·2026
Same journal

ATG9A is an essential host factor for parechovirus RNA replication.

Journal of virology·2026
See all related articles

Inhibiting AP-1 (activator protein-1) transcription factor activity suppressed oncogene-induced cellular transformation. The Fra-2/c-Jun heterodimer is crucial for this transformation, highlighting AP-1

Area of Science:

  • Molecular Biology
  • Oncology
  • Cellular Biology

Background:

  • Activating protein-1 (AP-1) is a transcription factor implicated in cellular processes.
  • Understanding AP-1's role in oncogene-induced cellular transformation is crucial for cancer research.

Purpose of the Study:

  • To investigate the necessity of endogenous AP-1 activity for cellular transformation mediated by various oncogenes.
  • To identify specific AP-1 components involved in oncogenic transformation.

Main Methods:

  • Utilized transdominant inhibitory fos (supfos-1) and jun (supjun-1) mutants in chicken embryo fibroblasts (CEF).
  • Assessed suppression of cellular transformation by monitoring reversion to normal morphology and anchorage-dependent growth.
  • Analyzed expression and activity of AP-1 components in oncogene-transformed CEF.

Related Experiment Videos

Main Results:

  • supfos-1 and supjun-1 efficiently suppressed transformation induced by fos/jun family oncogenes, v-src, v-yes, v-fps, c-Ha-ras, and c-raf.
  • Suppression was specific, as CEF transformed by v-ros or v-myc did not revert.
  • v-src, c-Ha-ras, and c-raf transformation elevated Fra-2 and c-Jun expression, with Fra-2 hyperphosphorylation observed.
  • The Fra-2/c-Jun heterodimer was identified as the primary driver of elevated AP-1 DNA-binding activity in transformed cells.

Conclusions:

  • Endogenous AP-1 activity is essential for cellular transformation induced by a range of oncogenes.
  • The Fra-2/c-Jun heterodimer plays a critical role in oncogene-induced cellular transformation.