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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
[Utilization of a system with application of a new media for detecting dermatophytes]
J Kwaśniewska1, A Glowacka, E Rubaj-Dudek
1Katedra Biologii i Parazytologii Lekarskiej AM, Lodzi.
Abstract:
Previously proposed system with application of new media was applied for differential diagnostics of dermatophytes. Evaluation concerned: 1. Direct preparations (KOH with glycerol, fluid with lactic acid and cotton blue), 2. Cultures on Sabouraud medium with CCG, Littman agar, agar supplemented with casein hydrolysate, erythritol and albumin, Christensen medium and 2% glucose agar, 3. Results of hair enticement test, 4. Clinical changes in Wood lamp. It was found that percentage of positive results (35.2 +/- 7.95%) was significantly lower (p < 0.001) than in culture when compared with results obtained from direct preparations. On erythritol medium strains from Trichophyton genus were isolated which was not the case on the Sabouraud medium. Christensen medium and agar containing 2% of glucose are most reliable in differentiating T. mentagrophytes and T. rubrum.
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