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Isolation and function of human and pig islets

S Takei1, M Teruya, A Grunewald

  • 1Department of Medicine, University of California, Irvine 92717.

Pancreas
|March 1, 1994
PubMed
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Researchers developed a reliable method to isolate functional pig and human islets for potential transplantation. This technique yields high-purity islets, demonstrating reproducible viability and secretory function in both species.

Area of Science:

  • Endocrinology
  • Transplantation Biology
  • Islet Biology

Background:

  • Islet transplantation is a promising therapy for type 1 diabetes.
  • Efficient and reproducible isolation of functional islets is critical for successful transplantation.
  • Porcine islets are a potential source for xenotransplantation due to their similarity to human islets.

Purpose of the Study:

  • To establish and validate a reproducible method for isolating and assessing the viability and function of both pig and human islets.
  • To compare the yield, purity, and functional characteristics of isolated islets from porcine and human pancreata.

Main Methods:

  • Modified automated method (Ricordi's) for islet digestion and isolation from 10 pig and 5 human pancreata.
  • Assessment of islet yield (islet equivalents per gram pancreas) and size distribution (50-200 microns).

Related Experiment Videos

  • Evaluation of islet purity (>90%) and in vitro function through perifusion studies measuring biphasic insulin release in response to glucose stimulation.

    • Main Results:

    • High yields of islet equivalents were obtained from both pig (335,190 +/- 79,345) and human (323,630 +/- 147,810) pancreata.
    • Islet preparations exceeded 90% purity, with the majority in the 50-200 micron size range.
    • Isolated islets from both species exhibited functional biphasic insulin release in response to glucose, confirming viability and responsiveness.

    Conclusions:

    • The modified automated method provides a reproducible means to isolate pure, viable, and functional islets from both pig and human pancreata.
    • The findings support the potential of this method for generating islets suitable for transplantation, particularly highlighting the utility of porcine islets.
    • This standardized isolation protocol is a significant step towards advancing islet cell-based therapies for diabetes.