Replication of border disease virus in ovine lymphocytes and monocytes in vitro

Adherent and non-adherent mononuclear cells obtained from the peripheral blood of normal sheep supported the in vitro replication of a non-cytopathic and a cytopathic strain of Border disease virus (BDV) with no apparent cytopathic effects. There was a significant rise in virus titres in adherent mononuclear cells (monocyte) and non-adherent (lymphocyte) cultures infected with both non-cytopathic and cytopathic strains of BDV 24 hours after inoculation. Peak virus titres of 5.36 log10 TCID50 ml-1 were recorded in adherent samples incubated for 48 hours while peak titres of 6.17 log10 TCID50 ml-1 were recorded in lymphocyte culture after 72 hours of incubation. Both the non-cytopathic and the cytopathic strains of BDV produced significantly higher titres in non-adherent (lymphocyte) cultures than in adherent (monocyte) cultures (P < 0.001) but the replication in adherent cells was faster than in nonadherent cell cultures. The addition of virus on both types of mononuclear cell cultures had no effect on cell viability but it had a significant inhibitory effect on the blastogenic responses of lymphocytes to phytohaemagglutinin.