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Bikunin in rat plasma, lymph and bile

A Słota1, M Sjöquist, M Wolgast

  • 1Institutionen för medicinsk och fysiologisk kemi, BMC, Uppsala, Sweden.

Biological Chemistry Hoppe-Seyler
|February 1, 1994
PubMed
Summary

Bikunin, a protease inhibitor, readily enters lymph, suggesting extravascular functions. Its distribution differs significantly from inter-alpha-inhibitor, indicating distinct roles beyond the bloodstream.

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Area of Science:

  • Biochemistry
  • Proteomics
  • Physiology

Background:

  • Bikunin is a protease inhibitor found in plasma, existing as free bikunin or complexed with inter-alpha-inhibitor.
  • Its role in blood is unclear due to weaker protease binding compared to other inhibitors.
  • Emerging evidence suggests bikunin has crucial functions outside the vascular system.

Purpose of the Study:

  • To investigate the extravascular distribution of bikunin.
  • To quantify free and complexed bikunin concentrations in plasma, lymph, and bile.
  • To compare the extravascular transport of bikunin and inter-alpha-inhibitor.

Main Methods:

  • Quantification of total and free bikunin concentrations in plasma, lymph, and bile.
  • Acid precipitation was used to separate free bikunin from its complex with inter-alpha-inhibitor.
  • Analysis of bikunin and inter-alpha-inhibitor ratios in isolated rat liver perfusate.

Main Results:

  • The lymph/plasma ratio of free bikunin (0.5) was higher than expected for its size, indicating significant extravascular presence.
  • The bile/plasma ratio for bikunin (0.02) was comparable to other proteins of similar size.
  • Inter-alpha-inhibitor showed higher vascular endothelium passage but restricted bile transfer compared to free bikunin.

Conclusions:

  • Bikunin demonstrates a notable capacity to enter extravascular spaces, particularly lymph.
  • The distinct distribution patterns of free bikunin and inter-alpha-inhibitor suggest specialized physiological roles.
  • Free bikunin is cleared from blood more rapidly than its complex with inter-alpha-inhibitor, supporting extravascular functions.

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