[Comparative study on cytotoxicity of lymphocytes from different tissues in laryngeal cancer]

PBL, TdLNL and TIL from twelve laryngeal cancer patients were cultured with rIL-2 for five to seven days and developed into LAK cells and TILs. The killing activities of LAK cells and TILs against K562 cells were measured by the method of 3H-TdR Incorporation. The results were as follows. After activation in vitro by rIL-2, PBL, TdLNL and TIL augmented in volume, became irregular in shape, and displayed all karyokinetic characteristics. Activated TILs showed cytotoxic activities against tumor cells; TdLNL isolated from the tumor-draining lymph-nodes were in large quantity. TdLNL could be cultured with rIL-2 alone for a long period, it may be the best way to harvest large quantity of LAK cells. LAK cells, from PBL and TdLNL had the same killing activities against K562 (P > 0.05). TIL activated by rIL-2 in short period also displayed killing activities against K562, but was less potent than LAK cells from PBL and TdLNL (P < 0.05). Lymphocytes of different sources could be effectively induced into LAK cell. On the basis of our experiments, the TdLNL could be used as a best source of LAK cells in the adoptive immunotherapy for laryngeal cancer.