A mycobacteriology proficiency testing program using simulated sputum specimens

Area of Science:

• Medical Laboratory Science
• Clinical Microbiology
• Public Health Surveillance

Background:

• Proficiency testing is crucial for ensuring laboratory accuracy in mycobacteriology.
• The New York State Department of Health has a long-standing program for evaluating laboratory performance.
• Changes in specimen preparation methods have occurred over time to meet increased demand.

Purpose of the Study:

• To assess the effectiveness of the New York State proficiency testing program in mycobacteriology.
• To evaluate laboratory performance in isolating and identifying mycobacteria.
• To identify factors influencing laboratory performance within the program.

Main Methods:

• Simulated sputum specimens containing Mycobacterium tuberculosis and other mycobacteria were used.
• Specimens were prepared using skimmed milk and granulated hog gastric mucin bases.
• Pseudomonas aeruginosa was added to simulate clinical specimen contamination.

Main Results:

• Overall laboratory performance in mycobacteriology has improved due to the proficiency testing and educational initiatives.
• Laboratories processing over 200 specimens annually consistently demonstrated superior performance.
• The program effectively determines a laboratory's ability to isolate and identify acid-fast bacilli.

Conclusions:

• The New York State proficiency testing program successfully enhances laboratory diagnostic capabilities in mycobacteriology.
• High-volume laboratories exhibit better performance, suggesting a need for continued support and training for all participants.
• The program's evolution in specimen preparation has supported its ongoing utility and impact on public health.