Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pulsatile insulin secretion from isolated human pancreatic islets

P Marchetti1, D W Scharp, M Mclear

  • 1Islet Transplantation Center, Washington University School of Medicine, St. Louis, Missouri.

Diabetes
|June 1, 1994
PubMed
Summary

Human pancreatic islets exhibit pulsatile insulin secretion regulated by an intrinsic pacemaker. This periodicity remains stable despite changes in glucose levels or other secretagogues, indicating its robustness.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Opioid prescribing patterns in trauma patients: assessing the impact of injury and treatment factors.

ANZ journal of surgery·2025
Same author

Expanded Characterization of a Hemi-Body Shielded Göttingen Minipig Model of Radiation-induced Gastrointestinal Injury Incorporating Oral Dosing Procedures.

Health physics·2018
Same author

Pilot Study of Radiation-induced Gastrointestinal Injury in a Hemi-body Shielded Göttingen Minipig Model.

Health physics·2018
Same author

24-hour profile of serum sclerostin and its association with bone biomarkers in men.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2017
Same author

Comparative environmental tolerances of threatened delta smelt (Hypomesus transpacificus) and introduced wakasagi (H. nipponensis) in an altered California estuary.

Oecologia·2017
Same author

[Diabetes and reticular macular dystrophy].

Journal francais d'ophtalmologie·2015

Area of Science:

  • Endocrinology
  • Cell Biology
  • Metabolic Regulation

Background:

  • Insulin secretion from the pancreas occurs in pulses.
  • The specific location and function of the pacemaker controlling human insulin periodicity are unknown.

Purpose of the Study:

  • To investigate the site and characteristics of the pacemaker regulating pulsatile insulin secretion in human pancreatic islets.

Main Methods:

  • Human pancreatic islets were isolated from organ donors.
  • Islets were perifused with varying glucose concentrations and secretagogues.
  • Insulin secretion was measured and analyzed for periodicity using cycle detection techniques.

Main Results:

  • Pulsatile insulin secretion was confirmed with a periodicity of approximately 10 minutes.

Related Experiment Videos

  • Increased glucose concentration significantly enhanced insulin secretion amplitude and overall release, but not periodicity.
  • Glucagon, arginine, and tolbutamide increased insulin release and amplitude without altering periodicity.

    • Conclusions:

    • A pacemaker regulating pulsatile insulin secretion is located within human pancreatic islets.
    • This pacemaker's periodicity is stable and unaffected by glucose levels or other secretagogues, suggesting inherent regulation.