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Cloning and sequence determination of the gene coding for the pyruvate phosphate dikinase of Entamoeba histolytica
E Saavedra-Lira1, R Pérez-Montfort
1Departamento de Microbiologia, Instituto de Fisiología Celular, UNAM, México D.F., México.
Gene
|May 16, 1994
Abstract:
We isolated three overlapping clones from a DNA genomic library of Entamoeba histolytica strain HM1:IMSS, whose translated nucleotide (nt) sequence shows similarities of 51, 48 and 47% with the amino acid (aa) sequences reported for the pyruvate phosphate dikinases from Bacteroides symbiosus, maize and Flaveria trinervia, respectively. The reading frame determined codes for a protein of 886 aa.