Related Experiment Video
Updated: Aug 2, 2026

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
Published on: January 6, 2016
Sequence of a human cDNA encoding phosphatidylinositol transfer protein and occurrence of a related sequence in
S K Dickeson1, G M Helmkamp, L R Yarbrough
1Department of Biochemistry, University of Kansas Medical Center, Kansas City 66160-7421.
Abstract:
Phosphatidylinositol (PtdIns) transfer protein (PtdInsTP) is a phospholipid transfer protein that has been detected in all mammalian tissues examined. It catalyzes the transfer in vitro of PtdIns and phosphatidylcholine between membranes in a number of natural and artificial membrane systems and may be involved in secretion in vivo. In previous studies, we isolated and sequenced a cDNA encoding a rat PtdInsTP. A rat cDNA probe was used to isolate clones from a lambda gt11 human testis cDNA library which encoded full-length human PtdInsTP. The cDNA sequence defines a 270-amino-acid, 31.8-kDa protein whose sequence shares 98.9% identity to that of rat, making it one of the most conserved proteins known between the two species. DNA blot hybridization studies suggest that there may be more than one gene encoding this protein in humans. A comparison of rat and human PtdInsTP cDNAs revealed strong sequence similarity (88 and 84%) in portions of the corresponding 5'- and 3'-untranslated regions (UTR) of the rat and human mRNAs.
More Related Videos
07:26Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
Published on: October 15, 2016
08:07Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Published on: July 26, 2019
Related Concept Videos
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Evolutionary Relationships through Genome Comparisons
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Eukaryotic Evolution
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
IP3/DAG Signaling Pathway
Three-Domain System of Life