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Characterization of IS905, a new multicopy insertion sequence identified in lactococci
1Department of Genetics and Microbiology, AFRC Institute of Food Research, Colney, Norwich, United Kingdom.
Journal of Bacteriology
|June 1, 1994
Summary
IS905, a mobile genetic element in Lactococcus lactis, is a 1313 bp insertion sequence. It contains a transposase gene and promoter-like sequences, showing similarity to IS256 elements.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- IS905 is a multicopy insertion sequence found in Lactococcus lactis.
- Insertion sequences are mobile genetic elements that can alter host genomes.
Purpose of the Study:
- To characterize the IS905 insertion sequence from Lactococcus lactis.
- To identify the functional components and potential regulatory elements of IS905.
Main Methods:
- Sequence analysis of the IS905 element.
- Identification of open reading frames and potential regulatory regions.
- Comparison with known insertion sequence families.
Main Results:
- IS905 is 1313 base pairs long and flanked by imperfect inverted repeats.
- It encodes a putative transposase of 391 amino acids.
- Potential promoter-active sequences were identified at one end of IS905.
- IS905 exhibits sequence homology to the IS256 class of insertion elements.
Conclusions:
- IS905 is a distinct insertion sequence with characteristics of the IS256 family.
- The presence of a transposase and potential promoter elements suggests IS905 is a mobile and potentially active genetic element.