Related Experiment Videos
Prenatal exclusion of paternity by PCR-FLP analysis of VNTR
E Arroyo1, F García-Sánchez, F Gómez-Reino
1Laboratoire de Histocompatibilidad, Centro de Transfusión, de la Communidad de Madrid, Spain.
Journal of Forensic Sciences
|March 1, 1994
Abstract:
A prenatal paternity test was requested by a 30-year-old woman. Variable number of tandem repeat (VNTR) systems were used for DNA analysis by means of amplification and electrophoresis followed by ethidium bromide staining or Southern blotting and oligonucleotide hybridization. Exclusion of paternity could be established on the basis of the great polymorphism and heterozygosity indexes of these genetic systems. This rapid method presents several advantages in contrast with other recombinant DNA techniques such as HLA class II oligotyping or RFLP.