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[An alternatively spliced form of the human thyrotropin receptor transcript]
1First Department of Internal Medicine, Nagasaki University School of Medicine.
Nihon Rinsho. Japanese Journal of Clinical Medicine
|April 1, 1994
Summary
Researchers cloned an alternatively spliced human thyrotropin receptor (hTSHR) mRNA variant. This smaller hTSHR cDNA-I encodes a truncated protein that may function as a TSH binding protein.
Area of Science:
- Molecular Biology
- Endocrinology
- Genetics
Context:
- Thyrotropin receptor (TSHR) plays a crucial role in thyroid hormone regulation.
- Alternative splicing of mRNA transcripts is a common mechanism for generating protein diversity.
- Previous detection of an alternatively spliced hTSHR mRNA in human thyroid cells.
Purpose:
- To clone and characterize an alternatively spliced form of the human thyrotropin receptor (hTSHR) mRNA.
- To determine the coding potential and potential function of the truncated TSHR protein.
Summary:
- An alternatively spliced hTSHR mRNA variant, hTSHR cDNA-I, was successfully cloned from human thyroid cells.
- This cDNA is approximately 1 kb and encodes a 253-amino acid protein, representing the N-terminal 60% of the extracellular domain.
- Sequence analysis suggests the presence of an unidentified intron within the cDNA-I.
Impact:
- The cloned truncated TSHR-I protein may be secreted.
- This secreted form could potentially act as a soluble TSH binding protein.
- Understanding this variant contributes to the broader knowledge of TSHR function and regulation.